Mahesh Sharma

MAHESH SHARMA

OBJECTIVE

To be a part of professional organization where I could apply and hone my acquired skills, to work the fullest of my potentials and knowledge in a challenging and intellectually stimulating environment in the field of biology and computational biology.

EDUCATIONAL QUALIFICATION

·         M.Sc, 2012 (Forensic science), Anglia Ruskin University, Cambridge, England (UK).

·         M.Sc, 2008 (Bioinformatics), Sikkim Manipal University.

·         B.Sc, 2005 (Microbiology + Pharmachemistry), Maharaja Ranjit Singh College. DAVV – INDORE

·         12^th , 2001 from SVH School – INDORE (M.P. Board)

·         10^th ,1998 from Govt. H school – Ujjain  (M.P. Board)

WORK EXPERIENCE

Working as a Forensic Expert & Investigator at Sparkle Institution of Forensic Science India from 1-Oct-2012 to till dated.

PUBLICATIONS, MAMBERSHIP AND PROFESSIONAL QUALIFICATION:

Sharma, M., Method development for analysis of Amphetamine-type drugs using GC-MS: Forensic Toxicology & drugs Abuse. 1. Germany: LAP Lambert Academic Publishing, 2013-10-25.Print & https://www.lap-publishing.com/catalog/details//store/gb/book/978-3-659-45267-3/method-development-for-analysis-of-amphetamine-type-drugs-using-gc-ms.

Sharma, M., a project report on pharmacophore modeling for leukmia. 1. 1. Indore: International Science Congress Association (ISCA), 2013. 1-99. eBook. <http://www.isca.co.in/PHARMA_SCI/pharma-sci-master.php>.

Sharma, M., Method development for analysis of Amphetamine-type stimulants using GC-MS. International Science Congress Association (ISCA) souvenir. -ISC-2012-9FS-06, PN: 165.

Fellow Member of SIFS INDIA.

Fellow Member of International Science Congress Association (ISCA).

DOEACC “A” level (PG-Level) in Bioinformatics Delhi.  (A unit of DOEACC Society,  An autonomous body  of Deptt. of Information  Technology, Ministry of Communications & Information Technology, Govt. of India) one paper left.

SUBJECTS STUDIED

Forensic science

Evidence Collection and Management (30 credits), Mastering Forensic Evidence (30 credits), Mastering Forensic Analysis (30 credits), Specialised Topics (15 credits), Research Methods and Preparation (15 credits), Masters Research Project (60 credits)

These are mostly a combination of laboratory and court reports (including witness statements), oral presentations and closed examinations. Some essay and report writing was required. The research project is by submission of a dissertation (20000 words) and oral presentation.

Experimental Forensic

Fingerprints analysis, hair analysis, drugs analysis, paint analysis, fiber analysis, documents analysis, foot prints, body fluids, blood pattern analysis using microbial, spectroscopic and mechanical technique such as, FTIR, GC-MS comparison microscope, low and high power microscope.

In addition of crime scene management, in a group we visit crime scene, collect evidence then this evidences was analyzed in laboratory and finally witness statement was presented in moot court.

Bioinformatics:

Data Base for Bioinformatics & Computing, Biological System & Bioinformatics, Windows Application, MS-Office, HTML, Multimedia, Static & Probability, Computational Method for Sequence Analysis, Biological Data Bank & Analysis, (OOPS), Perl for bioinformatics, VB, Drug Design &Gene Therapy, Plant Genomics, JAVA, Photoshop, Flash, Dream Weaver, Data Warehousing & Data Mining, Numerical Method & Optimization Tech, Emerging areas in Bioinformatics. 

Pharmachemistry:

TLC, HPLC, GLC, paper chromatography & column chromatography. Drugs preparation: - syrup, lotion, Drops crystallization. Drugs activity, QSAR, MM, ADME, IP, BP, Body Receptor, Na, K, Mg,-Channels, Chemical Isomerism.

Microbiology:

Soil Microbiology, Food Microbiology, Water Microbiology, Waste Water Treatment, Air Microbiology, Fundamentals of Genetics, DNA Replication and Gene Structure, Mutation, Genetic Recombination, Infection, Immune System , Immune Response, Antigens and Antibodies, Antigen and Antibody Reactions,  Fundamentals of Industrial Microbiology, Fermentor Design, Scale up, Industrial production, Bioassays, Quality Cont, Colorimetry and Spectrophotometry, Separation Techniques, Microorganisms in Agriculture, Geomicrobiology,  Pharmaceutical, Biotechnology,  Food from Microbes, Advanced Microbiology, Regulation of Gene Activity, Genetic Engineering, Gene Cloning, Techniques in Molecular Biology, Applications and Biohazards of Genetic Engineering

Experimental Microbiology:

Isolation of microorganisms by Sector Plate/Pour plate method, Differential staining, Special staining, Isolation of microorganisms from water / sewage / food / curd / canker/soil, Qualitative estimation of carbohydrates / proteins / lipids, Total count of RBC/WBC/Differential count of WBC/Hb estimation, Antigen-antibody reactions – Widal /VDRL, Isolation of mutants by replica plating technique/gradient plate technique, Identification of medically important organisms Staphylococcus / Streptococcus, E.coli ,  Urine analysis / Antibiotic sensitivity testing / Gradient Plate Technique,  Qualitative and Quantitative analysis of water/food/milk/sewage,  Microbial assay of Antibiotics/ Isolation of industrially important microbes/Paper Chromatography/TLC,  Isolation of bacterial/fungal DNA, Quantitative estimation of DNA/RNA, Isolation of Rhizobium

PROJECT & WORKSHOP

·         M.Sc. Forensic. Project: Method development for analysis of Amphetamine types stimulants using GC-MS

Abstract: A gas chromatography–mass spectrometry (GC–MS) method was developed and validated for the concurrent requirement and quantification of d-amphetamine sulphate, methamphetamine (MA), Ibuprofen, Piperazine hexahydrate, 1-(4-methoxyphenyl) piperazine (pMeOPP), 1-(4-Trifluoromethylphenyl) piperazine (4-TFMPP), 1-Benzylpiperazine (BZP) and 1-(2- methoxyphenyl) piperazine (oMeOPP), using Quinoline as an internal standard. All this abused drugs was purchased by chemical supplier. Different dilution preparation was calculated then specific amount of volume was used for different methods. After solutions were evaporated to dryness, they were derivatized using 50, 100, 150 and 200 µL pentafluoropropionic acid anhydride (PFPA) at 80^oC for 15, 30, 45 and 60 minutes. Time optimisation and derivatisation optimisation give very good results at 150 µL volume of PFPA with 80^oC temperature and 30 minutes duration of heating time. In addition as a second derivatisaing agent N,O-bis(trimethylsilyl)-trifluoroacetamide and trimethylchlorosilane (BSTFA:TMCS) were used at 70^oC for 30 minutes and analyzed by GC–MS.  The linear range was 0.025–0.5 mg/mL for all drugs, with the coefficients of determination (R²).  The limits of detections (LODs) and the limits of quantifications (LOQs) for each analyte were lower than 0.0111 mg/mL and 0.0317 mg/mL. The method was proved and is also suitable for the simultaneous detection and quantification of all selected drugs.

·         Project at BIOMED informatics, drug discovery and clinical research center, medwin hospitals in Hyderabad: Molecular Targeting against leukemia.

Abstract: Chromic myelogenus leukemia (CML) is characterized by the Philadelphia (Ph) chromosome and bcr/abl gene rearrangement which occurs in pluripotent hematopoietic progenitor cells expressing the c-kit receptor tyrosine kinase (KIT). Alternative splicing is a crucial mechanism for generating protein diversity. Different splice variants of a given protein can display different and even antagonistic biological functions. Therefore, appropriate control of their synthesis is required to assure the complex orchestration of cellular processes within multicellular organisms. One of the most exciting developments in cancer research in recent years has been the clinical validation of molecularly targeted drugs that inhibit the action fo pathogenic tyrosine kinases. The clinical validation of these “first-generation” tyrosine kinase inhibitors has been the prelude to a second wave of advances in molecular targeting that is expected to further change the way we classify and treat cancer. Efforts are now being directed at identifying the tumor subtypes and patients who will benefit the most from the drugs. Agents directed against new molecular targets are also being explored.

AIMs: Homology modeling of tyrosine kinase and Screening of compound library for tyrosine kinase inhibitors

·         M.Sc. Bioinformatics Project: Pharmacophore Modeling in LEUKEMIA Disease

Abstract: Three genetically linked leukemic genes are selected from the chromosomes (ABL1 chro-9, AFF1 chro-4, MKL1 chro-22). From these genes we find out some interest Protein having more than four ligands, For Leukemia disease a best suitable Pharmacophore Model was prepared with the help of Ligandscout 1.03 Tool. If any drug can bind with this Pharmacophore then we can have 100% treatment of genetically linked LEUKEMIA. In this whole project study, we have refered to NCBI, TIGR, EBI, HPRD, and SWISS-PROT for data base.

·         B.Sc. Project: Design of Fully Working Fermentor Model:

In this 2 liter fermentor model we can incubate Organism, add antifoaming agent, temperature control and have a couple of impeller to rotate. It uses 220 volt power supply. In a final Project of B.Sc. Microbiology.  

·         Attended workshop on “Biological sequences analysis and application in crop improvement” at G.B.Pant University (Uttarakhand)  Nov16-17,2007

·         Developed an Offline Web site For DOEACC final Project.

·         Six month DOTNET training at Alien SoftNet Technologies Pvt. Ltd.

TECHNICAL SKILLS

AREA OF INTEREST

My area of interest is not particular because during the journey of my education, whatever I earned I want to apply in my work skills according to needs. But I can focused on Crime scene investigator, Fingerprints analysis, hair analysis, drugs analysis, paint analysis, fiber analysis, documents analysis, foot prints and blood pattern analysis. Software developer in DOTNET Technology. Computer Aided Drug Designing, Molecular Modeling, Computational Biology, Immunology, Recombinant DNA Technology, Genomics & Proteomics, Metabolic pathways, Gene Sequence Analysis, Protein Sequence Analysis, Discovery of genuine drugs for Cancer using Bioinformatics Tools, Phylogenetic Analysis, Virtual Screening and Docking.

PERSONAL PARTICULARS